Influence of Intestinal Strangulation Release on Ischemia-reperfusion Injury in Sprague Dawley Rats

Ahmad Yani,1 Dorothy Dorothy,2 Rizky Amaliah1

1. Department of Surgery, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
2. Department of Surgery, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Correspondence to: Dorothy Dorothy,MD; Email:

Received: 9 March 2020; Revised: 31 July 2020; Accepted: 7 September 2020; Available online: 2 November 2020


Background: In intestinal ischemia, reperfusion towards the injured intestine can cause further injury to the intestine itself and to remote organs. This research aimed to evaluate the influence of intestinal strangulation release (SR) before resection on the intestine outside margin of the strangulated intestine compared with subjects without intestinal strangulation release (WSR). Methods: Fourteen male Sprague Dawley rats were subjected to strangulation of one loop of the distal ileum for 4 h. In the SR group, the strangulated intestine was released for 5 min and then resected for necrotic parts. In the WSR group, the strangulated intestine was immediately resected WSR. The control group received a sham laparotomy. Four hours after the second laparotomy, the animals were sacrificed, and intestinal samples were taken for histomorphological analysis and measurement of intestinal malondialdehyde (MDA) level. Results: The injury on the histomorphological intestinal mucosa and intestinal MDA level were insignificantly higher in the SR group than in the WSR group (p>0.05). Conclusion: Intestinal SR before resection causes more tissue injury and oxidative stress on the intestine outside the strangulation section, but the difference is not statistically significant.

Keywords: Ischemia-reperfusion injury, Intestinal ischemia, Intestinal strangulation release, Malondialdehyde, Intestine injury
Ann Afr Surg. 2021; 18(2): 90-95
Conflicts of Interest: None
Funding: None
© 2021 Author. This work is licensed under the Creative Commons Attribution 4. 0 International License.


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