Thyroid cancers in nodular goitres in Northwestern Nigeria
S.T. Edino, FMCS, FWACS, FICS, A.Z. Mohammed, FMCpath, O. Ochicha, FMCpath and S.A. Malami,
FMCpath, Departments of Surgery and Pathology, Bayero University, Kano, Nigeria
Dr. Edino ST., PMB 3452, Kano, Nigeria, Email: firstname.lastname@example.org
Background: Cancer is an occasional incidental finding in nodular goitres, but there has been no formal study on this disease in our centre which is located in an endemic goitrous region.
Aims and Objectives: To determine the prevalence and pattern of malignant growths in multinodular goitres.
Methodology: This is a seven year (2000-2006) retrospective study of all histologically diagnosed malignancies within nodular goitres at Aminu Kano Teaching Hospital, Kano.
Results: There were 160 multinodular goitres during the study period, out of which 24 (15.0%) had histologically diagnosed cancer and one out of the thirteen patients with solitary thyroid nodule (7.6%) had carcinoma. The ages of the patients with carcinoma ranged from 16 to 65 years, with a mean age of 38.8 years. Eighteen (72%) were females, and 7(28%) were males. Six out of the 25 cases of carcinoma were detected preoperatively by fine needle aspiration cytology. Well differentiated follicular carcinoma was the predominant histological type in 13 (52%) cases, followed by papillary in ten (40%), medullary carcinoma in one (4%) and anaplastic carcinoma in one (4%) patient.
Conclusion: One-seventh of nodular goitres in our centre harboured malignancy, and follicular carcinoma was the prevalent histological type. This is consistent with the findings elsewhere in endemic goitrous regions. Ultrasound guided fine needle aspiration would improve preoperative diagnosis and guide appropriate surgical management.
Nodular goitres are common causes of thyroid gland enlargement requiring surgical excision (1-3). Although these lesions are generally benign, several studies have documented occasional malignancies within these supposedly non-neoplastic nodules. The reported frequency of cancer in nodular goitre varies widely from four to 20% and it is generally thought to be more common in Solitary thyroid nodule than in Multinodular goitres (4-7). That cancer may arise within nodular goitre is not entirely surprising given the established epidemiological association between iodine -deficiency induced endemic goitre and follicular carcinoma (8,9). Nodular goitres are derived from longstanding/sporadic goitres due to sustained mitogenic stimulation of thyroid follicular cells by TSH (8). Different sub-populations of follicular cells with variable response to TSH proliferative signals soon emerge. It is this differential growth rate of different populations of follicular cells that is largely responsible for the characteristic nodularity of the disorder (8).
Several studies have demonstrated that the varied proliferative rates of different follicular cell populations in nodular goitre are due to clonal genetic mutations particularly affecting TSH signalling (8,10). Mutations in TSH signaling pathway are also known to be involved in follicular adenoma (11). Thus it can be hypothesized that gradual acquisition of further mutations in cancer related genes via tumour progression can lead to malignancy in nodular goitre. RAS, PAX8, and RET are among the cancer genes that have been implicated in malignant transformation of thyroid follicular cells (8). Nodular goitre may therefore be considered a neoplastic lesion.
Kano, in Northern Nigeria, falls within the iodine deficiency endemic goitre zone, so it is pertinent to evaluate and ascertain the frequency of malignancy in nodular goitres in our locality.
Materials and methods
This is a retrospective study of all surgically excised nodular goitres at Aminu Kano Teaching Hospital (AKTH), Kano, Nigeria between January 2000 and 2006. The specimens were submitted to our histo-pathology laboratory for histo-diagnosis. Biodata (age, sex, distribution of thyroid lesions and histological type of thyroid cancer) were collated from the patients` case folders and histopathological records. All the patients were pre-operatively evaluated by fine needle aspiration cytology (FNAC) and thyroid function tests. Biopsy specimens were fixed in 10% formol saline then processed into paraffin embedded sections and stained with haematoxylin and eosin. Histology slides were retrieved and reviewed by the consultant histo-pathologists for histological features of malignancy. The results are presented in tables.
During the study period, 184 patients had thyroidectomy for various thyroid diseases. Based on intra-operative and histo-pathological findings, the lesions were classified as simple multi-nodular goitre in 160 (87.0%) patients, solitary thyroid nodule in 13 (7.1%), toxic goitres in ten (5.4%), and Hashimoto’s thyroiditis in one (0.5%), as shown in Table 1. Thyroid carcinoma was detected in 25 (13.5%) out of the 184 thyroid lesions; twenty four of these was found in the 160 cases of multinodular goitres (15%), and one out of the 13 cases of solitary thyroid nodule (7.6%) had a focus of cancer. Carcinoma was detected pre-operatively in six out the 25 cases (24.0%) by fine needle aspiration cytology. Of the patients with carcinoma, 18 (72%) were females and seven (28%) males with ages ranging from 16 to 65 years with a mean of 38.8 years.
The histological types of the thyroid carcinoma are shown in Table 2. Well differentiated follicular carcinoma of the thyroid was the predominant type in 13 (52.0%) cases, papillary carcinoma in ten (40.0%), medullary carcinoma in one (4.0%), and anaplastic carcinoma in one (4.0%). No lymphoma was recorded in this study. The mean age+sd of the patients with follicular carcinoma was 42+14.5 years while it was 38+16.0 years for papillary carcinoma. Males had more papillary carcinoma in 57.1% (four out of seven cases), and out of the 18 females, 11 (61.1%) had follicular carcinoma, six (33.3%) papillary carcinoma, and one (5.6%) had medullary carcinoma (Table 3).
The overwhelming majority of multinodular goitres (MNGs) are benign and the diagnosis is mainly clinical. However the primary challenge in the management of non-functioning thyroid nodules is the exclusion of malignancy preoperatively by fine needle aspiration cytology (FNAC) with or without ultrasonography (12). This procedure is limited by sampling error and expertise in the interpretation of cytological findings (13). Sampling errors occasioned by the huge size of goitres seen in our centre could explain the inability to pre-operatively detect clinically unsuspected malignancy by FNAC in majority of cases. Moreover the predominant histological type which is follicular carcinoma is difficult to differentiate from follicular adenoma by cytological means. FNAC under ultrasound guidance or use of ultrasound guided core needle biopsy of a dominant nodule could enhance diagnosis and efficacy of the procedure (12,14). Despite negative FNAC, nodular goitres can still harbour carcinoma as found in this study (1-7). Therefore the key to diagnosis is careful definitive histopathological examination of resected thyroid tissues.
The risk of cancer in MNG is thought to be low compared to solitary thyroid nodules (STN) (15); but this is debatable (7,16). The prevalence of cancer in published series varies widely from 4%-17% in MNG (5) and 9%-25% in STN (6).
The findings in this study are comparable to other published reports (2,4,6,7). The reasons for the marked variation in nodular goitre cancer rates are not always clear. Epidemiological studies have demonstrated that the incidence of cancer in MNG is higher than in general population (4). Therefore the risk of malignancy in multinodular goitres should not be underestimated. Our findings are also in agreement with the assertion that in African zones of endemic goitre, the risk of cancer is much higher in MNG than in solitary thyroid nodules (2).
There is regional variation in the incidence rates and histopathological types of thyroid cancers. The incidence of thyroid malignancy recorded in this study is comparable to others (2,3,17) but much lower figures have been reported elsewhere (18). Majority of our patients are middle aged females, which is in keeping with other reports from our geographical area (17,19), but in Caucasians it occurs in the 4th to 5th decade (5). This lower age is probably related to endemicity of nodular goitres in our subregion (17,19). The most frequently encountered malignant thyroid tumour is papillary carcinoma especially in areas where iodine intake is adequate (4-7,17), however in endemic goitre zones, the follicular variant is more common, due most probably to persisting iodine deficiency (2,3). In contrast to a previous study from this centre (1), more cases of papillary carcinoma have been recorded. This changing pattern may be related to socioeconomic and environmental factors (17). Thyroid lymphoma was not recorded, while medullary and anaplastic carcinomas were uncommon in this report as in others (2,3,17,19). It was not known whether the case of medullary carcinoma in this study was sporadic or familial as the patient was lost to follow up after surgery.
This study has shown that the risk of malignancy in MNG is significant and the predominant histological type in our subregion remains the follicular variant.
Edino S.T., Mohammed A.Z. and Ochicha O. Thyroid gland diseases in Kano. Postgrad. Med. J. 2004; 11(2): 103-106.
Nmadu P. and Mabogunje O.A. The risk of cancer in endemic multinodular goitres. Afr. J. Med. 1991; 37(8): 242-244.
Lawal O., Agbakwuru A., Olayinka O.A., et al. Thyroid malignancy in endemic multinodular goitres: prevalence, pattern and treatment. J. Surg. Oncol. 2001; 27(2): 157-161.
Gandoffi P.P., Frisina A., Raffa M., et al. The incidence of thyroid cancers in multinodular goitres: a retrospective analysis. Biomed. 2004; 75(2): 114-117.
Koh K.B.H. and Chang K.W. Carcinoma in multinodular goitre. J. Surg. 1992; 79: 266-267.
McCall A., Jarosz H., Lawrence A.M., et al. The incidence of thyroid cancer in solitary thyroid nodules and in multinodular goitres. 1986; 100(6): 1128-1132.
Al- Saleh M.S. and Al-Kattan K.M. The incidence of cancer in multinodular goitre in Saudi Arabia. R. Coll. Surg. Edinb. 1994; 39: 106-108.
Maitra A. and Abass A.K. Thyroid gland in: Kumar V., Abass A.K., Fausto N. Robins and Contrans pathologic basis of disease. Elsevier Saunders Philadelphia. 2005 pg 1174-1182.
Franceschi S. Epidemiologia del carcinoma della tiroide. In Miani P eds.II carcinoma della toroide. Pisa: Pacini Editore, 1992; 13-29.
Apel R.L. Clonality of thyroid nodules in sporadic goitre. Mol. Pathol. 1995; 4: 113-121.
Rodien P. Activating mutations of TSH receptor. Endorinol. 2003; 12-16.
Kojic A.S., Halbauer M. and Tomic B.H. Importance of fine needle aspiration cytology in the detection of tumours within multinodular goitres. 2204; 15(4): 206-211.
Abu-Eshy S.A., Khan A.R., Khan G.M., et al. Thyroid malignancy in multinodular goitres. R. Coll. Surg Edinb. 1995; 40: 310-312.
Nicholas J.S., Laurence H.B. and John W.B. Ultrasound guided core-needle biopsy of the thyroid gland. 2003; 226: 827-832.
Sykes D. The Solitary thyroid nodule. J. Surg. 1981; 68: 510-512.
Sachmechi I., Miller E., Varatharajah R., et al. Pract. 2006; 6(1): 110-112.
Thomas A.O. and Ogunbiyi J.O. Thyroid cancers in Ibadan, Nigeria. East Afr. J. Med. 1995; 72(4): 231-233.
Anidi A.I., Ejeckam G.C., Ojukwu I., et al. Histological pattern of thyroid diseases in Enugu, Nigeria. E Afr. J. Med. 1983; 60(8): 564-570.
Ngadda H.A., Ojo A.S. and Adelusola K.A. A Histological analysis of thyroid diseases in Ile-Ife, Nigeria. Nig. Postgrad. Med. J. 2008; 15(1): 47-51.