Influence of Intestinal Strangulation Release on Ischemia-Reperfusion Injury in Sprague-Dawley Rat.

Ahmad Yani1, Dorothy Dorothy2, Rizky Amaliah1
1.Department of Surgery, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia

2.Department of Surgery, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Correspondence to: Dorothy Dorothy,MD; Email: dorothy.md@gmail.com

Abstract

Background: In intestinal ischemia, reperfusion towards the injured intestine can cause further injury to the intestine itself and remote organs. This research aims to evaluate the influence of intestinal strangulation release before resection on the intestine outside margin of the strangulated intestine, compared with subjects without intestinal strangulation release.

Methods: Fourteen male Sprague-Dawley rats were subject to strangulation of one loop distal ileum for four hours. On the strangulation release (SR) group, the strangulated intestine was released for 5 minutes and then resected for necrotic parts. On the without strangulation release (WSR) group, the strangulated intestine was immediately resected without strangulation release. The control group received sham laparotomy. Four hours after the second laparotomy, the animals were sacrificed, and intestinal samples were taken for histo-morphological analysis and measurement of intestinal malondialdehyde (MDA) level. 

Results: The histo-morphological intestinal mucosal injury and intestinal MDA level are insignificantly higher on the SR group compared to the WSR group (p>0.05). 

Conclusion: Intestinal strangulation release before resection causes more tissue injury and oxidative stress on the intestine outside the strangulation part, but the difference is not statistically significant

Keywords: Ischemia-reperfusion injury, intestinal ischemia, intestinal strangulation release, malondialdehyde, intestine injury

Ann Afr Surg. ****; **(*):***

DOIhttp://dx.doi.org/10.4314/aas.v*****

Conflicts of Interest: None

Funding: None

© 2020 Author. This work is licensed under the Creative Commons Attribution 4.0 International License.

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